Xinfan rat thromboxane B2 (TXB2) elisa kit introduction - Database & Sql Blog Articles

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Rat thromboxane B2 (TXB2) elisa kit

Introduction

Thromboxane (TXA2) is a type of prostaglandin that is produced by platelets and has platelet aggregation and vasoconstriction. In contrast to prostaglandins, the two are dynamically balanced to maintain vasoconstriction and platelet aggregation. TXA2 has a biological half-life of only 30 s and is rapidly converted to inactive thromboxane B2 (TXB2).

Rat thromboxane B2 (TXB2) elisa kit

Introduction

Rat thromboxane B2 (TXB2) elisa kit operation method

1. Dilute the antibody to 1-10 ug/ml with buffer. 0.1 ml was added to the reaction well at 4 ° C overnight. The next day, the solution in the well was discarded and washed 3 times with washing buffer.

2. Loading: Add 0.05 ml of the sample to be tested to the above-mentioned coated reaction well, and incubate at 37 ° C for 1 hour. Then wash (both blank wells, negative control wells and positive control wells).

3. Add the enzyme-labeled antibody: Add freshly diluted enzyme-labeled antibody (diluted by titration) to 0.05 ml in each reaction well. Incubate at 37 ° C for 0.5 to 1 hour and wash.

4. Add substrate liquid to develop color: Add 0.1 ml of temporarily prepared TMB substrate solution to each reaction well at 37 ° C for 10 to 30 minutes.

5. Stop the reaction: add 2M sulfuric acid 0.05ml to each reaction well.

6. Judgment of results: The results can be observed directly on the white background with the naked eye: the darker the color in the reaction well, the stronger the positive degree, the negative reaction is colorless or very light, according to the depth of the color, with "+", The "-" sign indicates. The OD value can also be measured: on the ELISA detector, at 450 nm (if the color is developed by ABTS, 410 nm), the OD value of each well is measured after zero adjustment of the blank control well, if it is greater than 2.1 times the OD value of the specified negative control. Is positive.

The advantages of our elisa kit: simple, fast, high sensitivity, specificity, cost-effective, and low sample requirements

1. The operation is simple and fast. All the steps, there are maps, so that you can be simple and clear, no need to delay the time for research steps.

2. High sensitivity. The enzyme is an organic catalyst, and a small amount of enzyme can induce a large amount of catalytic reaction, resulting in an observation color reaction phenomenon. Our kits quantify them at ug, ng, and pg levels.

3. Strong specificity. Specificity is derived from the selectivity of the antibody or antigen. The use of internationally renowned imported antibody antigens guarantees the quality of the kit.

4. High cost performance and stable quality.

5. The amount of sample required is small.

If you need to know the details of this product, please feel free to contact us.