Rubescens A test standard - Huaqiang Electronic Network

Properties: This product is a white crystalline powder; it is odorless and has a special odor.

Properties: This product is dissolved in solvents such as methanol, ethanol and acetone. It is slightly soluble in solvents such as petroleum ether and hardly soluble in water.

Check: Take this product

10mg

, adding methanol

10ml

After dissolution, the solution should be clear, colorless or yellowish.

Melting point: Take this product with phosphorus pentoxide as a desiccant, dry to a constant weight under reduced pressure, and take an appropriate amount to measure the melting point range by capillary method.

248

°C

-250

°C (Chinese Pharmacopoeia)

2005

Edition

Appendix VII

C

).

Loss on drying: Take this product, use phosphorus pentoxide as a desiccant, dry to a constant weight under reduced pressure, and reduce the weight by no more than

3%

(Chinese Pharmacopoeia

2005

Version 1 Appendix IX

G

).

Determination of content: according to high performance liquid chromatography (Chinese Pharmacopoeia)

2005

Edition Appendix VI

D

Determination, the normalization method must not be lower than

98%

, the external standard method is not lower than

95%

.

1.

Chromatographic conditions:

Column:

Hypersil C18 5μm 4.6mm×250mm

Mobile phase: methanol: water

=60

:

40

Flow rate:

0.8 ml/min

Detection wavelength:

238nm

2.

Preparation of standard solutions:

Precise weighing

8mg

Left and right Rubescens A reference substance

25ml

In the volumetric flask, use

15ml

Methanol is sonicated, cooled to room temperature and diluted to a constant volume until

25ml.

Shake well.

3.

Preparation of test solution:

Precision weighing is equivalent to Rubescensine A reference substance

8mg

Test sample

25ml

Volumetric flask

15ml

Methanol is sonicated, cooled to room temperature and then diluted to

25ml

Shake well, that is.

4.

Determination:

take

15μl

Methanol is injected into the liquid chromatograph to measure the blank, and then the reference solution and the test solution are accurately taken separately.

15μl

, injected into the liquid chromatograph

,

Record the peak area of ​​the reference and the test sample

.

5.

Calculation:

Wx = A1×C2×ws

A2×C1

Wx-------

Test content

C1-------

Test concentration

C2-------

Reference concentration

A1-------

Peak area of ​​test sample

A2-------

Reference peak area

Ws-------

Reference content

98%

meter

(

Our company

)

About Qingyun:

Nanjing Qingyun Biotechnology Co., Ltd. is located in Nanjing, the ancient capital of the Six Dynasties. The company is specialized in the production, sales and service. It is specialized in the research and development of plant extracts with plant extracts, active ingredient monomers and plant raw powder as the leading products. the company. The company has a first-class team of doctors and masters across the phytochemistry, Chinese pharmacy, biotechnology, natural medicinal chemistry, modern instrument analysis and advanced research and development and quality testing equipment in the domestic planting industry.

Qingyun Biological Ordering Instructions:

1,

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QQ

Orders and other methods. (When placing an order, you must inform the salesperson of the product name, content and quantity of the product)

2

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,

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